| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | D'ANNA, Celine | SR | Lecanto | 26.49 | FHSAA 3A District 6 |
| 2 | ALEXANDER, J'lae | SR | Lake Gibson | 26.32 | FHSAA 3A District 7 |
| 3 | JOHNSON, Amela | SO | Gateway (Kissimmee) | 26.21 | FHSAA 3A District 5 |
| 4 | WILSON, Chaze | SR | Hillsborough | 26.15 | FHSAA 3A District 8 |
| 5 | FINDLAY, Anaya | SO | King | 26.21 | FHSAA 3A District 7 |
| 6 | LITTLES, Kendria | SR | Leesburg | 26.30 | FHSAA 3A District 6 |
| 7 | LEWIS, Nehanda | JR | Land O'Lakes | 26.36 | FHSAA 3A District 6 |
| 8 | MOORE, Ida Rose | SR | Gulf | 27.00 | FHSAA 3A District 6 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KING, Ashanti | JR | Auburndale | 26.03 | FHSAA 3A District 7 |
| 2 | MILLER, Cache | JR | Jefferson | 25.92 | FHSAA 3A District 8 |
| 3 | WILSON, I'Asia | SR | Kathleen | 25.09 | FHSAA 3A District 7 |
| 4 | BURTON, Jenyia | SR | Jones | 24.04 | FHSAA 3A District 5 |
| 5 | THOMPSON, Cassie | SR | South Lake | 24.58 | FHSAA 3A District 5 |
| 6 | KULP, Diandra | SR | Spoto | 25.56 | FHSAA 3A District 8 |
| 7 | ELSTON, Iyana | JR | Blake | 25.95 | FHSAA 3A District 8 |
| 8 | HUET, Rylee | SR | Winter Springs | 26.09 | FHSAA 3A District 5 |