| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | McKeel | A | DNS | FHSAA 2A District 10 (4/23/2022) |
| 2 | Hardee | A | 54.68 | FHSAA 2A District 11 (4/19/2022) |
| 3 | Frostproof | A | DQ | FHSAA 2A District 10 (4/23/2022) |
| 4 | Berkeley Prep | A | 52.50 | FHSAA 2A District 9 (4/28/2022) |
| 5 | Port Charlotte | A | 52.80 | FHSAA 2A District 12 (4/21/2022) |
| 6 | Lemon Bay | A | 53.03 | FHSAA 2A District 12 (4/21/2022) |
| 7 | Davenport | A | 55.56 | FHSAA 2A District 10 (4/23/2022) |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Bishop Verot | A | 51.99 | FHSAA 2A District 12 (4/21/2022) |
| 2 | Sebring | A | 50.92 | FHSAA 2A District 11 (4/19/2022) |
| 3 | Parrish | A | 50.33 | FHSAA 2A District 11 (4/19/2022) |
| 4 | Lakewood | A | 49.89 | FHSAA 2A District 11 (4/19/2022) |
| 5 | Booker | A | 49.98 | FHSAA 2A District 12 (4/21/2022) |
| 6 | Holy Names | A | 50.79 | FHSAA 2A District 9 (4/28/2022) |
| 7 | Calvary Christian (Clr) | A | 51.26 | FHSAA 2A District 9 (4/28/2022) |
| 8 | Anclote | A | 52.29 | FHSAA 2A District 9 (4/28/2022) |