| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SLEDGE, Paul | FR | Mulberry | 24.07 | FHSAA 2A District 10 (4/23/2022) |
| 2 | DYER, Sytrevion | SO | Tenoroc | 23.37 | FHSAA 2A District 10 (4/23/2022) |
| 3 | SMITH, Dontavian | SR | DeSoto County | 23.30 | FHSAA 2A District 12 (4/21/2022) |
| 4 | POWELL, Lashawn | SR | Port Charlotte | 23.03 | FHSAA 2A District 12 (4/21/2022) |
| 5 | CAMPBELL, Levarian | SO | Clewiston | 23.05 | FHSAA 2A District 12 (4/21/2022) |
| 6 | TRIMBLE, Keon | SO | Clewiston | 23.30 | FHSAA 2A District 12 (4/21/2022) |
| 7 | ALPHONSE, Silas | SO | Discovery | 23.86 | FHSAA 2A District 10 (4/23/2022) |
| 8 | CALLAWAY, Quan'Tavios | SO | Lake Region | 24.08 | FHSAA 2A District 10 (4/23/2022) |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | EDWARDS, Ajani | SR | Jesuit (Tampa) | 23.01 | FHSAA 2A District 9 (4/28/2022) |
| 2 | MASON, Keveun | FR | Lake Placid | 22.89 | FHSAA 2A District 11 (4/19/2022) |
| 3 | BELL, Jamarives | SR | Gibbs | 22.64 | FHSAA 2A District 11 (4/19/2022) |
| 4 | VITORITT, Parker | SO | Parrish | 22.39 | FHSAA 2A District 11 (4/19/2022) |
| 5 | MCCLELLAN, Usir | SR | Clearwater Central Catholic | 22.54 | FHSAA 2A District 9 (4/28/2022) |
| 6 | MOSS, Von | FR | Parrish | 22.64 | FHSAA 2A District 11 (4/19/2022) |
| 7 | BARTON, Garrett | JR | Calvary Christian (Clr) | 22.99 | FHSAA 2A District 9 (4/28/2022) |
| 8 | QUARLES, Carlos | SR | Calvary Christian (Clr) | 23.03 | FHSAA 2A District 9 (4/28/2022) |