| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BARGER, Rachel | McKeel | 56.98 | FHSAA 2A District 10 |
| 2 | CERNIGLIA, Mackenzie | Bishop Verot | 53.95 | FHSAA 2A District 12 |
| 3 | CHERNIAK, Sophia | Lemon Bay | 52.64 | FHSAA 2A District 12 |
| 4 | BENITEZ, Alexi | Wesley Chapel | 51.30 | FHSAA 2A District 9 |
| 5 | HAFF, Kendal | Nature Coast | 52.40 | FHSAA 2A District 9 |
| 6 | WALLIN, Angelina | McKeel | 52.64 | FHSAA 2A District 10 |
| 7 | RIVERA MUNIZ, Sofia | Bonita Springs | 55.02 | FHSAA 2A District 12 |
| 8 | MURRAY, Catherine | Avon Park | 57.94 | FHSAA 2A District 10 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | LAMPRECHT, Gabiella | Parrish | 49.42 | FHSAA 2A District 12 |
| 2 | NEWBY, Arion | Calvary Christian (Clr) | 48.26 | FHSAA 2A District 11 |
| 3 | RODRIGUEZ, Paige | Tampa Prep | 48.04 | FHSAA 2A District 11 |
| 4 | GRAHAM, Alysia | Nature Coast | 47.36 | FHSAA 2A District 9 |
| 5 | WEEKLEY, Ellis | Berkeley Prep | 48.01 | FHSAA 2A District 11 |
| 6 | WILLIAMS , Chelsi | McKeel | 48.25 | FHSAA 2A District 10 |
| 7 | LACEY, Ashleigh | Cypress Creek (Wesley Chapel) | 49.14 | FHSAA 2A District 9 |
| 8 | ODOM, Maddie | Berkeley Prep | 50.19 | FHSAA 2A District 11 |