| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BYRD, Britton | Holy Names | 2:26.04 | FHSAA 2A District 11 |
| 2 | PAWLOSKI, Isabella | Calvary Christian (Clr) | 2:27.61 | FHSAA 2A District 11 |
| 3 | WEBER, Marissa | Clearwater Central Catholic | 2:29.02 | FHSAA 2A District 11 |
| 4 | GIBSON, Kendall | Citrus | 2:29.79 | FHSAA 2A District 9 |
| 5 | MAHAN, Lauren | Calvary Christian (Clr) | 2:29.80 | FHSAA 2A District 11 |
| 6 | REITZ, Julia | Bishop Verot | 2:29.84 | FHSAA 2A District 12 |
| 7 | GOMEZ , Cristal | Frostproof | 2:34.61 | FHSAA 2A District 10 |
| 8 | CIUNI, Samantha | Bishop Verot | 2:36.43 | FHSAA 2A District 12 |
| 9 | MARS, Jaclynn | Oasis | 2:37.98 | FHSAA 2A District 12 |
| 10 | THOMAS, Maddison | Bishop Verot | 2:38.03 | FHSAA 2A District 12 |
| 11 | ABBADESSA, Danika | Nature Coast | 2:41.27 | FHSAA 2A District 9 |
| 12 | MILLER, Alexa | Central (Brooksville) | 2:42.58 | FHSAA 2A District 9 |
| 13 | BYNUM, Willow | Wesley Chapel | 2:44.36 | FHSAA 2A District 9 |
| 14 | MANN, Alayna | McKeel | 2:46.12 | FHSAA 2A District 10 |
| 15 | NEWLAND, Savanna | McKeel | 2:54.04 | FHSAA 2A District 10 |
| 16 | MILLER , Emma | McKeel | 3:01.59 | FHSAA 2A District 10 |