| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ESQUILIN, Kennette | Avon Park | 50.78 | FHSAA 2A District 10 |
| 2 | PALMER, Brody | McKeel | 48.94 | FHSAA 2A District 10 |
| 3 | WILLIS, Reece | Lemon Bay | 44.04 | FHSAA 2A District 12 |
| 4 | FRIESZ, Joe | Calvary Christian (Clr) | 43.55 | FHSAA 2A District 11 |
| 5 | HARTWELL, Rashard | Lakewood | 43.55 | FHSAA 2A District 11 |
| 6 | WILSON, Anthony | Weeki Wachee | 46.05 | FHSAA 2A District 9 |
| 7 | ABUELOUF, Bilal | Lake Placid | 50.75 | FHSAA 2A District 10 |
| 8 | BOBO, Christian | Lake Placid | 56.11 | FHSAA 2A District 10 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HARRIS, Remedy | Wesley Chapel | 43.19 | FHSAA 2A District 9 |
| 2 | BURNETT, Canaan | Calvary Christian (Clr) | 42.00 | FHSAA 2A District 11 |
| 3 | BRADLEY, Caleb | Booker | 41.33 | FHSAA 2A District 12 |
| 4 | RICHARDS, David | Weeki Wachee | 40.34 | FHSAA 2A District 9 |
| 5 | ADAMS, Lutarus | Lakewood | 40.37 | FHSAA 2A District 11 |
| 6 | HILL, Chase | Bishop Verot | 41.37 | FHSAA 2A District 12 |
| 7 | TANNENBAUM, Jaden | Weeki Wachee | 42.80 | FHSAA 2A District 9 |
| 8 | AVERBECK, Noah | Cardinal Mooney | 43.40 | FHSAA 2A District 12 |