| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CARBONE, John | Central (Brooksville) | 1:58.95 | FHSAA 2A District 9 |
| 2 | HOLLIDAY, Daniel | Crystal River | 2:00.91 | FHSAA 2A District 9 |
| 3 | WEBER, Wolfgang | Berkeley Prep | 2:01.43 | FHSAA 2A District 11 |
| 4 | DOYLE, Mason | Berkeley Prep | 2:03.03 | FHSAA 2A District 11 |
| 5 | WEAVER, Austin | Gibbs | 2:03.60 | FHSAA 2A District 11 |
| 6 | BISHIR, Cody | Citrus | 2:03.88 | FHSAA 2A District 9 |
| 7 | ROWSELL, John | Cypress Creek (Wesley Chapel) | 2:05.45 | FHSAA 2A District 9 |
| 8 | DAVIS, Josh | Calvary Christian (Clr) | 2:06.17 | FHSAA 2A District 11 |
| 9 | BANKS, Cooper | Bishop Verot | 2:06.95 | FHSAA 2A District 12 |
| 10 | MORRISON, Isaac | McKeel | 2:06.95 | FHSAA 2A District 10 |
| 11 | BRANCH, Jason | LaBelle | 2:07.18 | FHSAA 2A District 12 |
| 12 | WILLS, Erikson | McKeel | 2:07.48 | FHSAA 2A District 10 |
| 13 | TUBBS, Grayson | Bishop Verot | 2:09.20 | FHSAA 2A District 12 |
| 14 | BLEM, Thomas | Lemon Bay | 2:12.25 | FHSAA 2A District 12 |
| 15 | MALDONADO, Emmanuel | DeSoto County | 2:23.78 | FHSAA 2A District 10 |
| 16 | WAGNER, Nevin | Lake Placid | 2:25.28 | FHSAA 2A District 10 |