| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ISAAC, Alvon | Villages | 22.87 | FHSAA 2A District 6 |
| 2 | GOMEZ, Leonardo | Dunnellon | 22.60 | FHSAA 2A District 6 |
| 3 | MCNAIR, Jamel | North Marion | 22.59 | FHSAA 2A District 6 |
| 4 | MACKEY, Jaden | Lincoln Park | 22.54 | FHSAA 2A District 8 |
| 5 | DAVIS, Nathaniel | Atlantic (PO) | 22.58 | FHSAA 2A District 7 |
| 6 | BAILEY, Gar'Mar | Cocoa | 22.59 | FHSAA 2A District 8 |
| 7 | GOLDWIRE, Ron'Darius | Santa Fe | 22.65 | FHSAA 2A District 5 |
| 8 | NELSON, Q'juan | Palatka | 22.87 | FHSAA 2A District 5 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | NEWMAN, Hayden | Palatka | 22.42 | FHSAA 2A District 5 |
| 2 | DENTON, TySheem | North Marion | 22.35 | FHSAA 2A District 6 |
| 3 | SMITH, Chandler | Bishop Moore | 21.96 | FHSAA 2A District 7 |
| 4 | BAPTISTE, Weston | Montverde Academy | 21.39 | FHSAA 2A District 7 |
| 5 | HAMILTON, Jaren | P. K. Yonge | 21.76 | FHSAA 2A District 5 |
| 6 | HOLMES, Xavier | Cocoa | 22.18 | FHSAA 2A District 8 |
| 7 | HOPKINS, Marcus | Cocoa | 22.36 | FHSAA 2A District 8 |
| 8 | MILLER, Solomon | Lake Highland Prep | 22.46 | FHSAA 2A District 7 |