| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | West Shore | A | 55.69 | FHSAA 2A District 8 |
| 2 | Eustis | A | 54.52 | FHSAA 2A District 6 |
| 3 | South Sumter | A | 54.34 | FHSAA 2A District 7 |
| 4 | Trinity Prep | A | 53.08 | FHSAA 2A District 7 |
| 5 | Dunnellon | A | 53.70 | FHSAA 2A District 6 |
| 6 | Lincoln Park | A | 54.49 | FHSAA 2A District 8 |
| 7 | Tavares | A | DNS | FHSAA 2A District 6 |
| 8 | Satellite | A | DNF | FHSAA 2A District 8 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Menendez | A | 52.33 | FHSAA 2A District 5 |
| 2 | Eastside | A | 52.12 | FHSAA 2A District 5 |
| 3 | Santa Fe | A | 51.61 | FHSAA 2A District 5 |
| 4 | Villages | A | 50.36 | FHSAA 2A District 6 |
| 5 | Bishop Moore | A | 51.45 | FHSAA 2A District 7 |
| 6 | Cocoa | A | 51.78 | FHSAA 2A District 8 |
| 7 | Palatka | A | 52.31 | FHSAA 2A District 5 |
| 8 | Lake Highland Prep | A | 52.37 | FHSAA 2A District 7 |