| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | FOSTER, Ava | Tavares | 27.67 | FHSAA 2A District 6 |
| 2 | MELCHIORRE, Michaela | Umatilla | 27.50 | FHSAA 2A District 6 |
| 3 | JONES, Aalaiah | Atlantic (PO) | 27.00 | FHSAA 2A District 7 |
| 4 | PARHAM, Angel | Cocoa | 26.80 | FHSAA 2A District 8 |
| 5 | GODBOLT, Reva | Interlachen | 26.96 | FHSAA 2A District 5 |
| 6 | THOMAS, Shunteria | Cocoa | 27.16 | FHSAA 2A District 8 |
| 7 | BROWN, Paris | Eastside | 27.61 | FHSAA 2A District 5 |
| 8 | KELLY, Emma | Mount Dora | 27.85 | FHSAA 2A District 6 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CLARK, Amber | Lincoln Park | 26.10 | FHSAA 2A District 8 |
| 2 | JENNINGS, Jaz'Myne | Montverde Academy | 25.54 | FHSAA 2A District 7 |
| 3 | TYSON, Liana | Cocoa | 25.44 | FHSAA 2A District 8 |
| 4 | BULFORD-TROTT , Keturah | Montverde Academy | 24.96 | FHSAA 2A District 7 |
| 5 | HENDERSON, Ke'Aria | North Marion | 25.28 | FHSAA 2A District 6 |
| 6 | REED, Zuriel | P. K. Yonge | 25.50 | FHSAA 2A District 5 |
| 7 | JONES, Kahdia | Atlantic (PO) | 25.79 | FHSAA 2A District 7 |
| 8 | MCBROOM, Destiny | Santa Fe | 26.36 | FHSAA 2A District 5 |