| Lane | Team | Squad | Seed | Note | 
|---|---|---|---|---|
| 2 | Lake Placid | A | 55.27 | 55.27 Lake Placid Meet %231 | 
| 3 | Sebring | A | 51.50 | 51.50 AP MEET %233 | 
| 4 | Lakewood | A | 49.96 | 49.96 Thomas Jet Jackson Invitational | 
| 5 | Parrish | A | 50.39 | 50.39 Manatee County Championships | 
| 6 | Hardee | A | 54.85 | 54.85 AP MEET %233 |