| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 212 | MARTINEZ, Giselle | FR | Lake Mary |
NT | |
| 3 | 508 | HEARD, Maya | SR | University (OC) |
1:12.08 | Seminole - Sanford Monday Meet #2 2025 |
| 4 | 388 | TWEEDIE, Madison | SR | Mount Dora Christian |
1:06.73 | Mount Dora Christian Invitational #1 |
| 5 | 243 | LANTZ, Caterina | FR | Lake Mary |
1:11.22 | Lake Mary Invitational 2025 |
| 6 | 218 | SELLERS, Adelaide | SO | Lake Mary |
1:15.14 | Lake Mary Opening Classic 2025 |
| 7 | 295 | GARRAUGHTY, Norah | SO | Lyman |
NT |