| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Seminole (Sanford) |
A | 8:13.12 | Lake Mary Invitational 2025 |
| 2 | Ridge Community |
A | DNS | Poinciana High School Relay Meet |
| 3 | Lake Mary |
A | 8:41.56 | Hagerty Track and Field Invitational 2025 |
| 4 | University (OC) |
A | 9:27.58 | Hagerty Track and Field Invitational 2025 |
| 5 | Trinity Christian (Deltona) |
A | 10:15.61 | Geneva Developmental Opener 2025 |