| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lake Mary |
A | 42.53 | Lake Mary Invitational 2025 |
| 2 | Spruce Creek |
B | 42.17 | Bob Hayes High School Invitational |
| 3 | Mainland |
A | 42.07 | FHSAA 3A Region 2 |
| 4 | Mainland |
C | 42.07 | FHSAA 3A Region 2 |
| 5 | Mainland |
B | 42.07 | FHSAA 3A Region 2 |
| 6 | Spruce Creek |
C | 42.17 | Bob Hayes High School Invitational |
| 7 | Spruce Creek |
A | 42.17 | Bob Hayes High School Invitational |
| 8 | Seminole (Sanford) |
A | DNS | FHSAA 4A Region 1 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | New Smyrna Beach |
A | 44.52 | Rockledge Raider Classic |
| 2 | East River |
C | 44.09 | Cocoa Invitational |
| 3 | East River |
B | DNS | Cocoa Invitational |
| 4 | West Orange |
A | DNS | West Orange - Dr. Baptiste Invitational 2025 |
| 5 | Menendez |
A | 42.82 | |
| 6 | East River |
A | 44.09 | Cocoa Invitational |
| 7 | Melbourne |
A | 44.24 | Astronaut Last Chance Meet 2024 |
| 8 | Astronaut |
A | 44.75 | Astronaut Last Chance Meet 2024 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Seabreeze |
A | 46.11 | Volusia/Flagler Fresh/ Sophomore Meet |
| 2 | Melbourne |
B | 46.00 | |
| 3 | Deltona |
A | 45.37 | Deltona Invitational 2025 |
| 4 | Atlantic (PO) |
A | 44.82 | Deltona Invitational 2025 |
| 5 | West Port |
A | DNS | |
| 6 | University (OC) |
A | 45.83 | Coach Ernest McPherson Invitational 2025 |
| 7 | New Smyrna Beach |
B | 46.00 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Menendez |
B | NT | |
| 4 | Melbourne |
C | 48.00 | |
| 5 | Eustis |
A | DNS |