| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lake Mary |
A | DNS | FHSAA 4A District 3 |
| 2 | West Orange |
B | DNS | Metro West Championships |
| 3 | Mainland |
A | 3:21.90 | FHSAA State Track & Field Championships (3A) |
| 4 | Mainland |
B | 3:21.90 | FHSAA State Track & Field Championships (3A) |
| 5 | Mainland |
C | 3:21.90 | FHSAA State Track & Field Championships (3A) |
| 6 | West Orange |
A | 3:23.46 | Metro West Championships |
| 7 | IMG Academy |
A | DNS | Charlotte Invitational |
| 8 | East River |
B | 3:30.28 | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Menendez |
A | 3:33.04 | George Janan Spruce Creek Invitational |
| 2 | Spruce Creek |
B | DNS | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| 3 | East River |
C | 3:30.28 | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| 4 | East River |
D | 3:30.28 | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| 5 | East River |
A | 3:30.28 | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| 6 | Spruce Creek |
A | 3:31.44 | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| 7 | New Smyrna Beach |
A | 3:31.66 | Tocoi Creek Toro Showdown 2025 (Approved Qualifier) |
| 8 | West Port |
A | DNS |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | West Port |
D | DNS | |
| 2 | University (OC) |
A | 3:48.89 | Coach Ernest McPherson Invitational 2025 |
| 3 | Melbourne |
A | 3:46.25 | 2025 MIHS Black & Gold Developmental Meet |
| 4 | Sheridan Hills Christian |
A | 3:41.35 | FHSAA 1A District 15 |
| 5 | Menendez |
B | NT | |
| 6 | Melbourne |
B | 3:46.25 | 2025 MIHS Black & Gold Developmental Meet |
| 7 | West Port |
C | DNS | |
| 8 | Deltona |
A | 3:54.05 | George Janan Spruce Creek Invitational |