| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | West Port |
B | DNS | Bob English Invitational 2025 |
| 2 | Lake Mary |
A | 8:00.02 | FHSAA 4A Region 1 |
| 3 | New Smyrna Beach |
A | 8:05.50 | FHSAA State Track & Field Championships (3A) |
| 4 | West Port |
A | 8:30.00 | |
| 5 | Sheridan Hills Christian |
A | 8:40.05 | Monsignior Pace |
| 6 | Spruce Creek |
A | 8:40.26 | FHSAA 4A Region 1 |
| 7 | East River |
A | 8:42.07 | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| 8 | Menendez |
A | DNS | |
| 9 | West Orange |
A | 9:02.00 | |
| 10 | University (OC) |
A | 9:16.07 | FHSAA 4A District 3 |
| 11 | Father Lopez |
A | 9:16.98 | FHSAA 1A Region 2 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Mainland |
A | 8:20.07 | 5-Star Conference Championship (FHSAA Sanctioned Meet) |
| 2 | East River |
B | DNS | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| 3 | Sheridan Hills Christian |
B | DNS | Monsignior Pace |
| 4 | Melbourne |
B | 8:40.12 | FHSAA 4A District 10 |
| 5 | Mainland |
B | 8:20.07 | 5-Star Conference Championship (FHSAA Sanctioned Meet) |
| 6 | Spruce Creek |
B | 8:40.26 | FHSAA 4A Region 1 |
| 7 | West Orange |
B | DNS | |
| 8 | Seabreeze |
A | 9:31.45 | 2025 Falcon Friday |
| 9 | Seabreeze |
B | 9:31.45 | 2025 Falcon Friday |
| 10 | Melbourne |
A | 10:10.00 | |
| 11 | Eustis |
A | DNS |