| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Seabreeze |
A | DNF | Bear Classic / True Team |
| 2 | Menendez |
A | 50.99 | |
| 3 | Mainland |
A | 49.66 | FHSAA 3A District 6 |
| 4 | West Port |
B | 48.62 | Father Pat Classic Sponsored by Trinity Catholic |
| 5 | American Heritage (DB) |
A | 48.96 | Lincoln Knowles Invitational 2024 (FHSAA Sanctioned) |
| 6 | Mainland |
C | 49.66 | FHSAA 3A District 6 |
| 7 | West Port |
A | 51.00 | |
| 8 | New Smyrna Beach |
A | 51.93 | Spruce Creek Developmental Meet #1 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | St. Johns Country Day |
A | 53.20 | FHSAA 1A District 6 |
| 2 | Astronaut |
A | DNS | Astronaut Invitational 2025 |
| 3 | Melbourne |
B | DNS | Viera High School Invitational 2 |
| 4 | Mainland |
B | 52.00 | |
| 5 | Melbourne |
A | DNS | Viera High School Invitational 2 |
| 6 | East River |
A | 52.46 | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| 7 | Spruce Creek |
C | 53.09 | |
| 8 | University (OC) |
A | 53.21 | Sanford - Seminole COACH ERNEST MCPHERSON INVITATIONAL 2024 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Menendez |
C | NT | |
| 3 | Spruce Creek |
A | 58.33 | |
| 4 | Spruce Creek |
B | 54.09 | |
| 5 | Menendez |
B | 55.00 | |
| 6 | Spruce Creek |
D | DNS |