| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | St. Johns Country Day |
A | 10:32.57 | 2025 Clay County Championship |
| 2 | New Smyrna Beach |
A | 10:41.60 | Tocoi Creek Toro Showdown 2025 (Approved Qualifier) |
| 3 | Sheridan Hills Christian |
A | 10:44.35 | Monsignior Pace 1 |
| 4 | West Port |
A | DNS | |
| 5 | Spruce Creek |
A | 11:01.00 | |
| 6 | Father Lopez |
A | 11:18.61 | FHSAA 1A District 6 |
| 7 | West Orange |
A | 11:26.00 | |
| 8 | Spruce Creek |
B | 11:30.00 | |
| 9 | East River |
A | 11:39.96 | East River Invitational |
| 10 | University (OC) |
A | DNS |