| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | New Smyrna Beach |
A | 4:11.29 | FSU Relays (High Schools) |
| 2 | Mainland |
B | 4:05.03 | FHSAA State Track & Field Championships (3A) |
| 3 | Mainland |
C | DNS | FHSAA State Track & Field Championships (3A) |
| 4 | American Heritage (DB) |
A | 4:03.39 | FHSAA State Track & Field Championships (1A) |
| 5 | IMG Academy |
A | DNS | |
| 6 | Mainland |
A | 4:05.03 | FHSAA State Track & Field Championships (3A) |
| 7 | West Port |
A | DNS | |
| 8 | Menendez |
A | 4:14.20 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | West Port |
D | DNS | |
| 2 | St. Johns Country Day |
A | DNS | FHSAA 1A District 6 |
| 3 | East River |
B | 4:20.88 | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| 4 | West Port |
B | DNS | |
| 5 | East River |
C | DNF | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| 6 | East River |
A | DNS | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| 7 | West Port |
C | DNS | |
| 8 | Sheridan Hills Christian |
A | 4:34.46 | FHSAA 1A Region 4 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Spruce Creek |
D | DNS | |
| 2 | Spruce Creek |
C | 4:50.09 | |
| 3 | Melbourne |
A | 4:40.43 | Viera High School Invitational 2 |
| 4 | University (OC) |
A | 4:37.11 | Sanford-Seminole Open 2025 |
| 5 | Spruce Creek |
A | 4:40.00 | |
| 6 | Spruce Creek |
B | 4:45.09 | |
| 7 | Seabreeze |
A | DNS | Deltona Invitational 2025 |
| 8 | Menendez |
B | NT |