| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 241 | MONTERO, Enrico | FR | Southeastern U. |
57.79 | UCF Knights Invite |
| 2 | 429 | ANDREWS, James | FR | Webber |
55.50 | |
| 3 | 303 | JOHNSON JR., Cedric | FR | Southeastern U. |
54.96 | UCF Knights Invite |
| 4 | 593 | ARISTIL, David | Unattached | 50.68 | ||
| 5 | 237 | LOPEZ, Vincent | SR | Southeastern U. |
53.42 | Knights Invite |
| 6 | 511 | MCHUGH, Colin | JR | Embry-Riddle |
55.25 | UNF East Coast Relays |
| 7 | 457 | ABELL, Chris | SR | Embry-Riddle |
55.55 | Embry-Riddle Running Elements Classic - Collegiate and Open |
| 8 | 455 | SCRABIS, Grant | FR | Embry-Riddle |
58.47 | Embry Riddle Blue and Gold Meet |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 153 | DOLTON, Aaron | FR | Flagler |
1:04.86 | Embry-Riddle Running Elements Classic - Collegiate and Open |
| 2 | 138 | HALL, Conner | FR | Flagler |
1:00.70 | The Spartan Classic |
| 3 | 197 | BELIZAIRE, Reuel | JR | Flagler |
59.55 | Annual Alice Coachman Invite |
| 4 | 140 | STANLEY, David | FR | Flagler |
58.47 | Annual Alice Coachman Invite |
| 5 | 52 | ECHEANDIA, Getulio | SR | Puerto Rican Masters | 59.00 | |
| 6 | 139 | JIMENEZ, Kruz | FR | Flagler |
1:00.12 | The Spartan Classic |
| 7 | 215 | RODRIGUEZ, Alexis | FR | Horizon Sunshine Academy | 1:01.94 | 2024 Montreat College Open |
| 8 | 611 | GULLEY, Taylor | SR | Unattached | NT |