| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Boone |
A | Alexander | Fouraker | Pullum | Sahaydak | 9:37.83 | FHSAA State Track & Field Championships (4A / Para-Athlete) |
| 2 | East Ridge |
A | Walker | Mustin | Foley | Warren | 9:42.51 | FHSAA 4A Region 1 |
| 3 | Horizon |
A | McBride | Brea | Frazer | McDonald | 10:12.75 | |
| 4 | Master's Academy (Oviedo) |
A | Gogley | Judy | Waters | Slater | 10:22.63 | |
| 5 | Windermere |
A | Lange | Kilmer | Ekebergh | Lack | 10:29.06 | Ocoee Invitational (Meet is Full) |
| 6 | Edgewater |
A | Spencer | Cronin | Roberts | Jackson | 10:31.51 | FHSAA 3A District 6 |
| 7 | Dr. Phillips |
A | Charir | Martin | Oliveira | Raymond | DNF | St. Cloud Invitational |
| 8 | West Orange |
A | Quayle | Baril | Zenkel | Gutierrez-Delgado | 11:10.41 | 11:10.41 Boone Invitational 2024 |
| 9 | Oviedo |
A | Cannaday | Cupit | Norena | Schnoor | NT |