| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | CALDWELL, Khamar | FR | Ocoee | 2:22.99 | RADD Track & Field Sunshine Showcase | 
| 2 | MCBRIDE, Keegan | SR | Windermere | 2:25.05 | Dr. Phillips Panther Invite | 
| 3 | SANCHEZ, Alberto | SO | Freedom (Orlando) | 2:25.62 | Freedom Developmental Track Meet 2023 | 
| 4 | DIFFENDAFFER, Easton | FR | Olympia | 2:28.20 | Windermere Meet of Champions Classic 2023 | 
| 5 | REY, Sebastian | JR | Foundation Academy | 2:28.87 | 2:28.87 Freedom High Open 2022 | 
| 6 | NEAL, Kenneth | JR | Ocoee | 2:30.00 | |
| 7 | BORI, Scott | SO | East Ridge | 2:32.19 | Early Hawk Invitational | 
| 8 | FINKELSTEIN, Oliver | FR | Winter Park | 2:34.89 | Cypress Creek (Orlando) Pre-Season Meet | 
| 9 | CLENDENIN, Charlie | SR | First Academy (Orlando) | 2:35.10 | Royal Invitational @ TFA 2021 | 
| 10 | GOMEZ, Lucas | JR | Maclay | 2:35.68 | Chiles Freshmen Sophomore Championship | 
| 11 | MANGO, Manuel | SO | Timber Creek | 2:36.21 | Lake Mary Opening Classic 2023 | 
| 12 | MONTALVAN, Michael | FR | East Ridge | 2:36.65 | Early Hawk Invitational | 
| 13 | LOPEZ, Juan | SR | Freedom (Orlando) | 2:44.69 | Early Hawk Invitational | 
| 14 | VAZQUEZ, Kaleeb | SO | South Lake | 2:44.87 | Early Hawk Invitational | 
| 15 | BERNAL, Sebastian | SO | Freedom (Orlando) | NT | |
| 16 | FALAHPOUR, Shawn | SO | Windermere | NT | |
| 17 | MONTALVAN, Jason | FR | East Ridge | NT | |
| 18 | OLINGER, Liam | FR | Foundation Academy | NT | |
| 19 | SCHREIBMAN, Jayden | SO | South Lake | NT | |
| 20 | SUNDERMEYER, Quin | FR | Foundation Academy | NT | |
| 21 | WILSON, Charles | FR | Maclay | NT | |
| 22 | ZHIHANG, Lin | FR | First Academy (Orlando) | NT | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | MUSSELWHITE, Noah | SR | Apopka | 1:52.33 | FHSAA Class 4A & Para Track & Field State Championships | 
| 2 | PETERSEN, Will | SR | Boone | 2:00.27 | FHSAA 4A Region 3 | 
| 3 | WOOD, Nolan | SR | Winter Park | 2:00.55 | 2:00.55 Trinity Prep - Saint's Invitational 2022 | 
| 4 | CANNELLA, Dylan | JR | Boone | 2:04.74 | Boone Invitational 2023 | 
| 5 | HERNANDEZ, Adams | JR | Cypress Creek | 2:05.00 | |
| 6 | MCKEE, Jaiden | SO | West Orange | 2:05.00 | |
| 7 | ASSEFA, Saafir | SR | Boone | 2:06.79 | Winter Park - Wildcat Open 2023 | 
| 8 | FARLEY, Lukas | JR | West Orange | 2:07.13 | 2:07.13 Metro West Championship | 
| 9 | ANTHONY, Collin | SR | Olympia | 2:07.52 | Kowboy Developmental Meet 1 | 
| 10 | ABBOTT, Caleb | SR | Winter Park | 2:08.26 | Trinity Prep - Saint's Invitational 2022 | 
| 11 | MAUCH, Lucas | SR | Maclay | 2:08.82 | Chiles Capital City Classic | 
| 12 | PRIETO IV, Pablo | FR | Timber Creek | 2:10.00 | |
| 13 | MATHEWS, Devin | FR | Ocoee | 2:13.62 | Winter Park - Wildcat Open 2023 | 
| 14 | TILUS, Ryan | JR | Cypress Creek | 2:14.25 | Boone Invitational 2023 | 
| 15 | OVERTON, Bowen | SO | Windermere | 2:14.57 | Windermere Meet of Champions Classic 2023 | 
| 16 | NWOKEOCHA, Dillan | SO | Cypress Creek | NT | |
| 17 | DIVRA, Jorey | JR | West Orange | 2:15.19 | Winter Park - Wildcat Open 2023 | 
| 18 | STEWART, Myles | FR | South Lake | 2:17.46 | Early Hawk Invitational | 
| 19 | PETERMAN, Gavin | FR | Timber Creek | 2:18.00 | |
| 20 | PARMENTER, Ethan | FR | Olympia | 2:18.19 | Kowboy Developmental Meet 1 | 
| 21 | ATCHISON, Nathanael | JR | First Academy (Orlando) | 2:20.47 | 2:20.47 Highlander Classic 2022 |