| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | WAPOLE, Wyatt | JR | Foundation Academy | NT | |
| 2 | CHAMBERS, Justin | FR | Boone | NT | |
| 3 | DUVAL, Ryan | FR | Freedom (Orlando) | NT | |
| 4 | CAMPBELL, Marc | SO | Maclay | NT | |
| 5 | DAVIS, Sawyer | SR | Maclay | NT | |
| 6 | ULLOA, Octavio | SO | Windermere | NT | |
| 7 | MANUEL, Garrson | SR | East Ridge | NT | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | NEMBHARD, Kyle | SO | Freedom (Orlando) | 29.28 | Early Hawk Invitational | 
| 2 | HENDERSON, Izaiah | SO | South Lake | 27.53 | George Jenkins - Bob English Invitational 2023 | 
| 3 | PATTERSON, Elisha | SR | East Ridge | 25.87 | Early Hawk Invitational | 
| 4 | JENKINS, Nathan | JR | Apopka | 25.68 | Mount Dora Developmental Meet #2 | 
| 5 | GARDNER, Sawyer | FR | First Academy (Orlando) | 25.78 | St. Cloud Invitational | 
| 6 | QUINTANA, Arturo | SO | Freedom (Orlando) | 26.19 | Freedom Developmental Track Meet 2023 | 
| 7 | RODGERS, Shamar | SO | South Lake | 28.40 | George Jenkins - Bob English Invitational 2023 | 
| 8 | WILLIS, Blake | SR | Boone | NT | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | MOATS-MAYNARD, Uriah | SO | South Lake | 25.36 | Freedom High Open 2022 | 
| 2 | KNIGHT, Jackson | SO | Winter Park | 24.70 | |
| 3 | HARRIS JR, Keith | FR | Timber Creek | 24.30 | |
| 4 | CLARKE, Jordon | SR | East Ridge | 24.04 | Wally Keller Invitational | 
| 5 | MARCELUS, Jeffery | SR | Jones | 24.20 | George Jenkins - Bob English Invitational 2023 | 
| 6 | TENCZAR, Jason | SR | First Academy (Orlando) | 24.53 | Cypress Creek (Orlando) Pre-Season Meet | 
| 7 | SPENCER, Noah | SO | Windermere | 24.99 | Dr. Phillips Panther Invite | 
| 8 | CAMPOS, Bryan | SO | Winter Park | 25.50 | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | GRAHAM, Kameron | SR | Boone | 23.96 | Boone Invitational 2023 | 
| 2 | MASON, Bradley | JR | First Academy (Orlando) | 23.83 | Cypress Creek (Orlando) Pre-Season Meet | 
| 3 | FERRER, Fabian | FR | Timber Creek | 23.56 | |
| 4 | SEIDE, Velson | SR | Olympia | 23.50 | Windermere Meet of Champions Classic 2023 | 
| 5 | CENESCAR, Esaie | SO | Cypress Creek | 23.50 | |
| 6 | ARCHER, Michael | JR | Apopka | 23.70 | |
| 7 | SANCHEZ-RODRIGUEZ, Denzel | JR | Winter Park | 23.90 | |
| 8 | JEAN, Daniel | JR | Foundation Academy | 23.97 | 23.97 Mount Dora Christian Invitational Meet #2 | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | ROGERS, Zephy | JR | Foundation Academy | 23.47 | 23.47 Mount Dora Christian Invitational Meet #2 | 
| 2 | GREEN, Raymond | SO | Ocoee | 23.40 | |
| 3 | HARRELL, Douglas | SR | Maclay | 23.05 | |
| 4 | CHERY, Kevin | SR | Ocoee | 22.90 | |
| 5 | BROWN, Keon | SR | Ocoee | 23.02 | Dr. Phillips Pre-Season Invitational | 
| 6 | MCCORMICK, Keandre | SO | West Orange | 23.10 | |
| 7 | NICHOLS, Dayveone | JR | Jones | 23.43 | George Jenkins - Bob English Invitational 2023 | 
| 8 | PAYNE, Lorenzo | JR | Apopka | 23.50 | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | WALKER, Romelo | JR | Olympia | 22.76 | |
| 2 | ROGERS, Henry | FR | Windermere | 22.55 | |
| 3 | FRIEDMAN, Cameron | SR | Cypress Creek | 22.48 | |
| 4 | RUDOLPH, Valentino | SR | West Orange | 21.09 | |
| 5 | GILLENS, Jayden | SR | Jones | 22.15 | |
| 6 | DAVIS, Emmanuel | JR | Timber Creek | 22.51 | 22.51 Metro East Conference Championships | 
| 7 | JEFFERSON, Davarrian | FR | Olympia | 22.58 | |
| 8 | DAVIS, Brandon | JR | West Orange | 22.90 |