| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | JORDAN, Ishaan | SO | Freedom (Orlando) | NT | |
| 2 | BANVILLE, Joaquim | FR | Windermere | NT | |
| 3 | VU, Hai | SR | Maclay | NT | |
| 4 | ALBEQUERQUE, David | FR | East Ridge | NT | |
| 5 | BILLINGS, Ashton | SO | Windermere | NT | |
| 6 | HAMPTON, Kaedon | SR | South Lake | NT | |
| 7 | HARDY, Justin | FR | East Ridge | NT | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | BUSTAMANTE, Franco | SO | Windermere | NT | |
| 2 | OLIVER, Elijah | FR | Boone | NT | |
| 3 | LIGHTNEAR, Gedont | FR | East Ridge | 1:02.27 | Early Hawk Invitational | 
| 4 | VAN DYKE, Atticus | SO | Winter Park | 57.50 | |
| 5 | COX, Aidan | SO | Freedom (Orlando) | 1:02.07 | Early Hawk Invitational | 
| 6 | BURDEN, Caleb | FR | Foundation Academy | 1:03.85 | Freedom Developmental Track Meet 2023 | 
| 7 | CAMPBELL, Marc | SO | Maclay | NT | |
| 8 | HAY, Jacob | SO | Boone | NT | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | KELLY, Colton | SO | Foundation Academy | 57.25 | Freedom Developmental Track Meet 2023 | 
| 2 | ELLIS, Jackson | SO | Winter Park | 56.81 | |
| 3 | KARSTAMATIS, Alejandro | FR | Winter Park | 56.50 | |
| 4 | CULVER, William | JR | Olympia | 56.15 | Windermere Meet of Champions Classic 2023 | 
| 5 | ROSENTHAL, Jesse | 8 | Foundation Academy | 56.17 | Freedom Developmental Track Meet 2023 | 
| 6 | PIERCE, Elliott | JR | First Academy (Orlando) | 56.55 | FHSAA 1A District 8 | 
| 7 | CORONA, Jose | FR | Freedom (Orlando) | 57.22 | Freedom Developmental Track Meet 2023 | 
| 8 | HARRIS, Keith | FR | Timber Creek | 57.43 | Lake Mary Opening Classic 2023 | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | WILSON, Rodney | JR | Timber Creek | 55.71 | Lake Mary Opening Classic 2023 | 
| 2 | RUTHERFORD, Ramone | SO | Apopka | 55.00 | |
| 3 | TELUSNORD, Jamaar | SR | Olympia | 53.64 | FHSAA 4A District 9 | 
| 4 | JONES, Stone | SR | Ocoee | 53.02 | |
| 5 | TREWIN, Andrew | SR | South Lake | 53.05 | Early Hawk Invitational | 
| 6 | MACHADO, John | FR | Cypress Creek | 54.00 | |
| 7 | YEPES, Sebastian | SR | First Academy (Orlando) | 55.66 | 55.66 Evans Trojans Relays | 
| 8 | SAINT-LOUIS, Evansky | SR | South Lake | 55.96 | Freedom High Open 2022 | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | SMITH, Jordan | SO | Apopka | 53.00 | |
| 2 | MCLEAN, Reese | SO | Apopka | 53.00 | |
| 3 | WILLIAMS, Solomon | JR | Timber Creek | 52.82 | Brian Jaeger Elite Classic 2022 | 
| 4 | ROBINSON, Jaheim | SR | Olympia | 52.30 | |
| 5 | TOLBERT, Daven | JR | West Orange | 52.50 | |
| 6 | JEFFERSON, Zion | SR | Boone | 52.87 | |
| 7 | WHITE, Christian | SO | Ocoee | 53.00 | |
| 8 | MCCASKILL, Caleb | SR | Jones | 53.00 | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | MOORE, Anyas | JR | Jones | 51.81 | 51.81 East River Invitational | 
| 2 | JEAN SIMON, Derrick | SR | Cypress Creek | 51.00 | |
| 3 | BROWN, Keon | SR | Ocoee | 50.20 | |
| 4 | FRIEDMAN, Cameron | SR | Cypress Creek | 49.50 | |
| 5 | DAVIS, Brandon | JR | West Orange | 50.20 | Windermere Meet of Champions Classic 2023 | 
| 6 | ALEXANDER, Terrel | JR | West Orange | 50.80 | |
| 7 | CLARK, Phillip | SR | First Academy (Orlando) | 51.46 | FHSAA 1A Region 2 | 
| 8 | FORINE, Branardo | JR | Jones | 52.00 |