| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | VAN DER RIET, Rahel | SR | Foundation Academy | 2:49.04 | Freedom Developmental Track Meet 2023 | 
| 2 | LUGO-RAMOS, Neely | SO | East Ridge | 2:49.21 | FHSAA 4A District 4 | 
| 3 | CASADO, liliana | SR | West Orange | 2:50.66 | Winter Park - Wildcat Open 2023 | 
| 4 | GUTIERREZ-DELGADO, Paula | SO | West Orange | 2:51.40 | Windermere Meet of Champions Classic 2023 | 
| 5 | MALDONADO, Lina | SR | East Ridge | 2:52.50 | Freedom Developmental Track Meet 2023 | 
| 6 | BERENS, Katie | SO | Apopka | 2:53.26 | FHSAA 4A District 4 | 
| 7 | RAHALI, Rania | JR | Freedom (Orlando) | 2:53.65 | Early Hawk Invitational | 
| 8 | GAUD, Liani | SO | Foundation Academy | 2:59.02 | FHSAA 1A District 7 | 
| 9 | RHODEN, Aniya | JR | Olympia | 3:01.56 | Cypress Creek (Orlando) Home Meet 1 | 
| 10 | DAVIS, Addison | JR | Foundation Academy | 3:07.10 | Freedom High Open 2022 | 
| 11 | SAINTLOT, Karlie | SR | East Ridge | 3:09.35 | Freedom Developmental Track Meet 2023 | 
| 12 | MORA, Jocelyn | JR | South Lake | 3:09.40 | 3:09.40 South Sumter High School Raider Invitational | 
| 13 | TREJO, Leah | FR | South Lake | 3:10.47 | Early Hawk Invitational | 
| 14 | CESAIRE, Celina | SO | Cypress Creek | 3:16.05 | Cypress Creek (Orlando) Pre-Season Meet | 
| 15 | TIRADO, Janielis | FR | Freedom (Orlando) | 3:35.60 | Freedom Developmental Track Meet 2023 | 
| 16 | MARTINEZ RUIZ, Reatchel | JR | Cypress Creek | NT | |
| 17 | DERISMA, Soleil | JR | Olympia | NT | |
| 18 | BALL, Mackenzie | SO | Winter Park | NT | |
| 19 | PRESLAR, Riley | SR | Timber Creek | NT | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | RUSSELL, Ziza | FR | Timber Creek | 2:23.19 | Boone Invitational 2023 | 
| 2 | ALEXANDER, Sabine | FR | Boone | 2:23.25 | Winter Park - Wildcat Open 2023 | 
| 3 | FROST, Aubrey | SO | Olympia | 2:25.33 | Windermere Meet of Champions Classic 2023 | 
| 4 | GROSS, Sarah | SR | Winter Park | 2:26.41 | 2:26.41 Dr. Phillips Panther Invite | 
| 5 | LACK, Ava | SO | Windermere | 2:27.00 | |
| 6 | KOEPPEL, Lillian | FR | Maclay | 2:28.07 | Jesse Forbes HS Invitational | 
| 7 | KOEPPEL, Susan | JR | Maclay | 2:29.75 | Chiles Freshmen Sophomore Championship | 
| 8 | THUMM, Payton | SO | Maclay | 2:31.30 | FHSAA 1A Region 1 | 
| 9 | FITOS, Kaelynn | JR | Windermere | 2:32.85 | The Elite 16 Track & Field Invitational | 
| 10 | BOURNE, Drayton | JR | Boone | 2:34.95 | Brian Jaeger Elite Classic 2022 | 
| 11 | LAUTERWASSER, Emma | FR | Cypress Creek | 2:35.00 | |
| 12 | SAHAYDAK, Layla | FR | Boone | 2:35.00 | |
| 13 | LOMAX, Mia | FR | Ocoee | 2:40.02 | |
| 14 | ZENKEL, Haven | FR | West Orange | 2:44.08 | Windermere Meet of Champions Classic 2023 | 
| 15 | DUDEK, Charlotte | SO | Windermere | 2:44.52 | FHSAA 4A District 9 | 
| 16 | THALMANN, Isabelle | SR | Ocoee | 2:45.36 | Apopka HS Pre-Season Meet | 
| 17 | ENGLEHART, Mia | SR | Freedom (Orlando) | 2:45.43 | FHSAA 4A District 9 | 
| 18 | SMITH, Alexis | SO | South Lake | 2:46.54 | Early Hawk Invitational | 
| 19 | CHRISTIE, Daisha | SR | Ocoee | 2:47.00 | |
| 20 | JACKSON, Sarah | SR | First Academy (Orlando) | 2:47.33 | 2:47.33 Highlander Classic 2022 |