| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | PIERRE, Jaslynn | JR | Winter Park | NT | |
| 2 | HOFFMAN, Kyla | FR | Foundation Academy | NT | |
| 3 | DAVIS, Nadia | FR | Freedom (Orlando) | NT | |
| 4 | THOMAS, Savannah | FR | East Ridge | NT | |
| 5 | BROCKMAN, Lilli | FR | Windermere | NT | |
| 6 | COOPER, Jayla | SO | South Lake | NT | |
| 7 | WIGGINS, Jazzlynn | SR | Jones | NT | |
| 8 | MAZZIOTTA, Ellie | 8 | Maclay | NT | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | ROMERO, Alejandra | FR | Freedom (Orlando) | 15.90 | Early Hawk Invitational | 
| 2 | HOLLIS, Jazmya | SO | South Lake | 15.17 | George Jenkins - Bob English Invitational 2023 | 
| 3 | MCCOY, Nyah | SO | West Orange | 14.26 | Windermere Meet of Champions Classic 2023 | 
| 4 | CHISHOLM, Nia | FR | Cypress Creek | 14.13 | |
| 5 | ZEISLOFT-ACREE, Madison | JR | First Academy (Orlando) | 14.18 | FHSAA 1A District 8 | 
| 6 | FERGUSON, Mya | FR | Timber Creek | 14.44 | Boone Invitational 2023 | 
| 7 | LOZADA DA SILVA, Anastasia | FR | First Academy (Orlando) | 15.19 | St. Cloud Invitational | 
| 8 | FOSTER, Valecia | SO | Olympia | NT | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | CRUZ FELICIANO, Angelina | FR | Cypress Creek | 14.00 | |
| 2 | WILLIAMS, Mykel | JR | Boone | 13.83 | FHSAA 4A District 9 | 
| 3 | GORDON, Jada | FR | Windermere | 13.70 | Windermere Meet of Champions Classic 2023 | 
| 4 | SMITH, Aniya | SO | West Orange | 13.63 | East River Invitational | 
| 5 | REID, Mariaya | SO | Jones | 13.68 | St. Cloud Invitational | 
| 6 | AUNGST, Chloe | FR | Apopka | 13.70 | |
| 7 | CALDERON, Aryana | SR | Timber Creek | 13.99 | Lake Mary Pre-Season 2022 | 
| 8 | MINCELY, Alexis | FR | Windermere | 14.06 | Windermere Meet of Champions Classic 2023 | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | BRYANT, Cianna | SR | Foundation Academy | 13.60 | FHSAA 1A District 7 | 
| 2 | ROBBINS, Marchele | FR | Boone | 13.55 | Boone Invitational 2023 | 
| 3 | RANKING, Ayana | JR | Apopka | 13.40 | |
| 4 | FLOWERS, Jolie | SR | Freedom (Orlando) | 13.32 | FHSAA 4A District 9 | 
| 5 | PIOLI, Isabella | FR | Timber Creek | 13.35 | Boone Invitational 2023 | 
| 6 | BENSON, Alexia | SO | East Ridge | 13.50 | Early Hawk Invitational | 
| 7 | MASSEY, Sydnee | JR | Olympia | 13.57 | Windermere Meet of Champions Classic 2023 | 
| 8 | PHILLIPS, Shila | FR | Winter Park | 13.63 | Boone Invitational 2023 | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | BERNARD, Patley | SR | Winter Park | 13.21 | St. Cloud Track Invitational | 
| 2 | OCASIO, Rayona | SO | Jones | 12.96 | FHSAA 3A Region 2 | 
| 3 | MANNING, Lakisha | SR | Boone | 12.88 | 12.88 Metro East Conference Championships | 
| 4 | DOWDY, Elizabeth | SO | Maclay | 12.76 | Jesse Forbes HS Invitational | 
| 5 | NEWTON, Jayla | FR | Ocoee | 12.80 | |
| 6 | COBB, Dontaesha | JR | Cypress Creek | 12.90 | |
| 7 | HENDERSON, Amaris | FR | Apopka | 13.20 | |
| 8 | CAMPBELL, Johanna | SO | West Orange | 13.23 | Brian Jaeger Elite Classic 2022 | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | CALDWELL, Destiny | SR | Ocoee | 12.67 | Brian Jaeger Elite Classic 2022 | 
| 2 | ROBERTS, Asia | SO | South Lake | 12.56 | FHSAA 3A District 5 | 
| 3 | POWELSON, Emily | SR | First Academy (Orlando) | 12.41 | FHSAA Class 1A Track & Field State Championships | 
| 4 | PETERS, Lee | FR | Foundation Academy | 12.22 | FHSAA Class 1A Track & Field State Championships | 
| 5 | BETHKE, Rachel | SR | Maclay | 12.24 | 2023 Chiles Track and Field Relays | 
| 6 | ROBINSON, Nauti'ka | SR | Olympia | 12.47 | FHSAA 4A Region 3 | 
| 7 | GREEN, Leneah | SO | Ocoee | 12.60 | Windermere Meet of Champions Classic 2023 | 
| 8 | NUMAN, Yamina | SR | East Ridge | 12.70 | 12.70 Lake- Sumter Championships |