| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Pine Ridge |
B | Quinones | Castro | Camacho | Rodriguez | NT | |
| 4 | Mainland |
B | Parks | Rucker | Thompson | Ciferri | DQ | |
| 5 | Deltona |
B | Ware | Lewis | Benjamin | Baldwin | NT | |
| 6 | University (OC) |
B | Elder | Santos | Cruz | Carter | 3:55.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Pine Ridge |
A | Alston | Genaro | Gavilanes | Hipster | 4:00.00 | |
| 2 | University (OC) |
A | Andrews | Burns | Kwamina | Swint | 3:49.58 | 3:49.58 Deland HS Big D Relays 2024 |
| 3 | Deltona |
A | Castro | Pierre | Becerra | Haughton | 3:49.58 | 2024 George Janan Spruce Creek Invitational |
| 4 | Mainland |
A | Wilmore | Wood | Berry | Curry | 3:23.00 | |
| 5 | Seabreeze |
A | Moore | Davis | Dayton | Lewis | 3:30.63 | FHSAA 3A District 6 |
| 6 | Trinity Christian (Deltona) |
A | Hudzik | Ponce-Arce | Thompson | Cruz | 4:08.82 | Geneva Developmental Opener 2025 |