| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 3 | 102 | ORR, Amya | SO | University (OC) |
22.34 | Deltona Invitational 2024 |
| 4 | 25 | BARR-MILLER, Nyrania | SR | University (OC) |
14.98 | FHSAA 4A District 3 |
| 5 | 53 | SUPERNAW, Julianna | JR | University (OC) |
20.60 | Deltona Invitational 2024 |
| 6 | 92 | BRYANT, Jazlyn | FR | University (OC) |
22.51 | Deltona Invitational 2024 |