| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Lake Weir | A | NT | ||||
| 2 | Olympia | A | Scott | Heald | Roundtree | Ardon | DNS |
| 3 | Father Lopez | A | Campos | Fulton | Stanberry | Ginnochetti | DNS |
| 4 | Mainland | A | Hawryluk | Nixon | Demarest | Hinson | 4:23.25 |
| 5 | DeLand | A | Neira | Nguyen | Bordeaux | Kersey | 4:25.00 |
| 6 | University (OC) | A | Pickens | Smith | Burgos Rosado | Arroyo Canales | 4:45.00 |