| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 | University (OC) | A | Elkind | Penna | Bradley | Ayende-Ortiz | 3:37.16 | 
| 2 | Spruce Creek | A | Davis | Mobley | Evans | Harris | 3:34.05 | 
| 3 | Olympia | A | Graham | Friedman | Satchell | Limon | 3:29.70 | 
| 4 | Mainland | A | Wood | Sawyer | Camara | Mcquire | 3:26.23 | 
| 5 | DeLand | A | Williams | Soto, Jr. | Edwards-Pla | Myrie | 3:27.00 | 
| 6 | Olympia | B | Boisrond | Montes | Anthony | Anthony | 3:32.00 | 
| 7 | Mainland | B | Washington | Shine | Yisrael | Cambell | 3:35.00 | 
| 8 | Cypress Creek | A | Lewis | Santos | Jimenez-Abreu | Torres Plaza | 3:39.51 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Lake Weir | A | DNS | ||||
| 3 | Father Lopez | A | Pardes | Fulton | Herdel | Gazzoli | DNS | 
| 4 | Atlantic (PO) | A | Russell | Preston | dixon | royal | 3:50.98 | 
| 5 | Atlantic (PO) | B | Rittman | furlonge | strickland | russell | 3:51.05 | 
| 6 | North Marion | A | Mestre | Greene | Johnson | Cardigan | DNS |