| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | North Marion | A | Taylor | Mestre | Arias | Beard | 1:32.55 |
| 2 | Spruce Creek | A | Davis | Harris | Mobley | Jeune | 1:30.07 |
| 3 | Olympia | A | Friedman | Graham | Thompson | Satchell | 1:27.80 |
| 4 | DeLand | A | Williams | Edwards-Pla | Myrie | Soto, Jr. | 1:26.00 |
| 5 | Atlantic (PO) | A | Preston | royal | dixon | Washington | 1:27.46 |
| 6 | Cypress Creek | A | Graciano | Watkins | Cenescar | Marcelus | 1:30.00 |
| 7 | Lake Weir | A | Cooper | Dawkins | Hamilton | Forshee | 1:30.98 |
| 8 | Atlantic (PO) | B | Rittman | Benezette | Johnson | furlonge | 1:36.05 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Cypress Creek | B | NT | ||||
| 2 | Spruce Creek | B | Sudesh | Price | Sampson | Ellis | NT |
| 3 | Mainland | A | Shine | Durden | Washington | Porter | NT |
| 4 | Father Lopez | A | Ginocchetti | Herdel | Sweeney | Mcduffie | DNS |
| 5 | University (OC) | A | Cook | Irwin | Williams | Henry | 1:41.00 |
| 6 | Olympia | B | Mills | Montes | Gonzalez | Boisrond | NT |