| Lane | Team | Squad | Seed | Note | 
|---|---|---|---|---|
| 1 | Lakeland | A | 8:00.55 | |
| 2 | McKeel | A | 8:23.76 | FHSAA Class 2A/Para-Athlete Track & Field State Championship | 
| 3 | George Jenkins | A | 8:40.31 | 8:40.31 Bob English Relays 2022 | 
| 4 | Ridge Community | A | 8:41.00 | |
| 5 | Lake Wales | A | 8:55.00 | |
| 6 | Mulberry | A | 9:43.81 | 9:43.81 Polk County Championship 2019 | 
| 7 | Frostproof | A | NT | |
| 8 | Santa Fe Catholic | A | NT | |
| 9 | Winter Haven | A | NT | |
| 10 | Discovery | A | NT |