| Lane | # | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|---|
| 2 | 632 | BENNETT, Jelaun | SR | Frostproof | 55.17 | Frostproof - Bulldog Invite 2022 | 
| 3 | 274 | MCKINNEY, Jamar | FR | Davenport | 50.20 | |
| 4 | 74 | POPE, Jacob | SR | Lakeland | 49.18 | Buffalo Invitational Presented by the Villages | 
| 5 | 353 | BULGER, Jon | 8 | Lakeland Christian | 49.64 | Tampa Bay Private Schools Track and Field Championship | 
| 6 | 96 | SANCHEZ, Steven | SR | Winter Haven | 51.69 | The RunMatanzas T&F Invitational 2022 | 
| 7 | 391 | DAVIS, Steven | SO | McKeel | 57.72 | Lakeland City Meet 2022 | 
| Lane | # | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|---|
| 1 | 427 | RODRIGUEZ, Jomar | JR | McKeel | 49.05 | Lakeland City Meet 2022 | 
| 2 | 102 | TULLIS, Marlon | FR | Winter Haven | 48.42 | Bob English Relays 2022 | 
| 3 | 110 | CAMPBELL, Tyshawn | SR | Winter Haven | 45.85 | Bob English Relays 2022 | 
| 4 | 29 | ESTRADA, Franeudi | SR | Lakeland | 41.53 | Lakeland City Meet 2022 | 
| 5 | 365 | ALLEN, Jacob | JR | Lakeland Christian | 44.33 | Tampa Bay Private Schools Track and Field Championship | 
| 6 | 33 | JOHNSON, Don'Ares | JR | Lakeland | 46.81 | The Bob English 2021 | 
| 7 | 286 | MCWORTER, Seth | JR | Davenport | 49.00 | Windermere Meet of Champions Classic 2022 | 
| 8 | 163 | BAKER, Donovan | SR | Ridge Community | 49.05 | George Jenkins Invite 2021 |