| Lane | # | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|---|
| 1 | 63 | THOMPSON, Trey | SR | Lakeland | 2:25.75 | Buffalo Invitational Presented by the Villages | 
| 2 | 316 | DENTON, Jadin | SO | Lakeland Christian | 2:26.16 | Jenkins Classic 2021 | 
| 3 | 150 | MORAN, Pedro | SO | Ridge Community | 2:27.97 | Poinciana Invitational | 
| 4 | 361 | DAVIS, Miles | SO | Lakeland Christian | 2:28.42 | Shorecrest Quad Meet | 
| 5 | 94 | KEY-HARRINGTON, Quran | SO | Winter Haven | 2:28.57 | Bob English Relays 2022 | 
| 6 | 170 | MERCADO GONZALEZ, Emmanuel | SR | Ridge Community | 2:28.57 | Poinciana Invitational | 
| 7 | 380 | MOXLEY, Jackson | SR | McKeel | 2:31.74 | George Jenkins Tri Meet | 
| 8 | 184 | MILLER, Sean | FR | Fort Meade | 2:32.89 | Lake Region Sprint/Hurdle Meet 2022 | 
| 9 | 160 | CASTINERIRAS, Ashe | FR | Ridge Community | 2:35.85 | Poinciana Invitational | 
| 10 | 260 | GONZALES, Sean | FR | Davenport | 2:37.32 | Dr. Phillips Panther Invite | 
| 11 | 629 | SANCHEZ, Juan | SO | Frostproof | 2:39.91 | Tohopekaliga Invitational 1 | 
| 12 | 404 | BIRGE, Jackson | 8 | McKeel | 2:40.64 | Lake Region Distance Carnival 2022 | 
| 13 | 486 | STRICKLAND, Coen | FR | Mulberry | 2:59.72 | Bob English Relays 2022 | 
| 14 | 300 | TOWNSON, Ronnie | FR | Santa Fe Catholic | 2:59.75 | Lake Region Sprint/Hurdle Meet 2022 | 
| 15 | 604 | VELEZ-MARTINEZ, Jovian | 6 | Frostproof | 3:00.37 | 6th Annual George Jenkins Invitational 2022 | 
| 16 | 506 | AGARD, Derek | JR | Mulberry | NT | |
| 17 | 558 | BILBREY, Jeremiah | 7 | Oasis Christian Academy | NT | |
| 18 | 579 | CARTER, Omega | SR | Discovery | NT | |
| 19 | 559 | SPENCER, Bailey | JR | Oasis Christian Academy | NT | |
| 20 | 589 | ST JENTE, Jesus | SO | Discovery | NT | |
| 21 | 581 | NIEVES, Josean | JR | Discovery | NT | 
| Lane | # | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|---|
| 1 | 706 | JOSEPHS, Brant | JR | Windermere Prep | 2:02.22 | |
| 2 | 544 | LANG, Preston | SR | Lake Gibson | 2:04.27 | Windermere Meet of Champions Classic 2022 | 
| 3 | 690 | ALLWOOD, Lorenzo | SO | Windermere Prep | 2:04.46 | Trinity Prep - Citrus League Championship 2022 | 
| 4 | 742 | HOLDEN, Patrick | SO | Windermere Prep | 2:04.55 | |
| 5 | 393 | WILLS, Erikson | JR | McKeel | 2:05.97 | Lakeland City Meet 2022 | 
| 6 | 455 | ARIAS-RUNYAN, Cristian | JR | Lake Wales | 2:12.00 | |
| 7 | 297 | WELLING, Daniel | SO | Santa Fe Catholic | 2:12.47 | Lake Region Sprint/Hurdle Meet 2022 | 
| 8 | 295 | LOVELY, James | SO | Santa Fe Catholic | 2:12.88 | Lake Region - Mike Williams Open 2022 | 
| 9 | 28 | DEJARNETTE, Quinn | SO | Lakeland | 2:13.46 | Buffalo Invitational Presented by the Villages | 
| 10 | 31 | WHEELER, Christopher | JR | Lakeland | 2:14.67 | Buffalo Invitational Presented by the Villages | 
| 11 | 225 | BAKER, Caden | FR | George Jenkins | 2:15.00 | |
| 12 | 217 | OTERO, Samuel | FR | George Jenkins | 2:15.00 | |
| 13 | 356 | SMITH, Jack | SR | Lakeland Christian | 2:15.51 | Shorecrest Senior Night | 
| 14 | 504 | SALCEDO, Kevin | SR | Mulberry | 2:19.00 | |
| 15 | 194 | SNELL, Jackson | SO | George Jenkins | 2:20.00 | |
| 16 | 528 | CAMPBELL, LeAndre | FR | Lake Gibson | 2:20.20 | Bob English Relays 2022 | 
| 17 | 81 | MIRANDA, Manuel | SO | Winter Haven | 2:21.08 | 6th Annual George Jenkins Invitational 2022 | 
| 18 | 478 | LENNON, Kaheim | SO | Lake Wales | 2:21.69 | Kowboy Invitational | 
| 19 | 102 | TULLIS, Marlon | FR | Winter Haven | 2:23.50 | Tiger Invitational | 
| 20 | 585 | PEDEREDUX, Tyreke | SO | Discovery | NT |