| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MACGILVRAY, Charles | Ponte Vedra | 53.46 | |
| 2 | DE LA TORRE, Cameron | Nease | 53.22 | Don Hall Invitational / St. John River Conference |
| 3 | STRATTON, Matthew | St. Johns Country Day | 53.00 | |
| 4 | REMISIEWICZ, Quinn | Ponte Vedra | 52.86 | Tocoi Creek Toro Showdown |
| 5 | OSBORNE, Joshua | Ponte Vedra | 52.92 | |
| 7 | SAULSBERRY, Jarron | Fleming Island | 53.35 | Don Hall Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HAMMONS, Tariku | Ponte Vedra | 52.78 | |
| 2 | DIXON, Omarr | Providence | 52.20 | Bishop Snyder Quad Meet |
| 3 | GILL, Dylan | Nease | 52.09 | FHSAA 4A District 2 |
| 4 | STOCKTON, Seth | Columbia | 53.08 | Oak Hall Mini Meet #3 |
| 5 | WEIGEL, Ryan | Bartram Trail | 51.71 | |
| 6 | STIMPHIL, Josber | Oakleaf | 52.20 | 52.20 Don Hall Invitational |
| 7 | BERRY, Brandon | Bartram Trail | 52.55 |