| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | FRASER, Alexandrea | Montverde Academy |
NT | 57.48 AAU Nationals Results https://fl.milesplit.com/athletes/10383761-alexandrea-fraser |
| 3 | SING, Trinity | Windermere Prep |
1:15.45 | Bishop Moore - Carl Friedland Invite 2025 |
| 4 | PADIN-CASTILLO, Abiana | Bishop Moore |
1:10.80 | Lake Highland - Highlander Classic 2025 |
| 5 | LAITINEN, Aili | Windermere Prep |
1:13.00 | Lake Highland - Highlander Classic 2025 |
| 6 | ZREIK, Elena | Windermere Prep |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | JIANG, Yingquian | Lake Highland Prep |
1:09.66 | Geneva Classic 2025 |
| 3 | ALICEA, Lauren | Lake Highland Prep |
1:09.35 | Lake Highland - Highlander Classic 2025 |
| 4 | PAREKH, Anisa | Lake Highland Prep |
1:08.82 | Hagerty Track and Field Invitational 2025 |
| 5 | TRACY, Tynan | Lake Highland Prep |
1:08.97 | Bishop Moore - Larry Kelly Invitational 2025 |
| 6 | JOHNSON, Alyssa | Windermere Prep |
1:09.57 | Bishop Moore - Carl Friedland Invite 2025 |
| 7 | BIRD, Makayla | First Academy (Orlando) |
1:09.75 | ROYAL INVITATIONAL @ The First Academy 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SUFFRAN, Engreed | Bishop Moore |
1:06.80 | 2025 Hawks on the Hill Invitational (Regional Qualifier) |
| 2 | FONES, Valen | First Academy (Orlando) |
1:01.54 | Lake Buena Vista Home Meet |
| 3 | LANDRI, Gianna | First Academy (Orlando) |
58.40 | Windermere Friday Night Lights |
| 4 | REED, Dasia | Montverde Academy |
54.88 | The Legends of Alachua County Invitational High School |
| 5 | DICKINSON, Ellise | Montverde Academy |
58.06 | Mount Dora Christian Invitational #1 |
| 6 | DE LA TORRE, Sofia | Bishop Moore |
1:00.75 | ROYAL INVITATIONAL @ The First Academy 2025 |
| 7 | CLAYTOR, Charity | First Academy (Orlando) |
1:02.52 | Windermere Friday Night Lights |
| 8 | CANTERO, Elena | Bishop Moore |
1:08.64 | Lake Highland - Highlander Classic 2025 |