| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | BISSONNETTE, Luke | First Academy (Orlando) |
NT | NT |
| 3 | STRESSER RIBEIRO, Rafael | Windermere Prep |
NT | 53.0 |
| 4 | O'CONNOR, William | Trinity Prep |
1:03.18 | ROYAL INVITATIONAL @ The First Academy 2025 |
| 5 | MANSILLA, Jack | Windermere Prep |
1:08.59 | Windermere Friday Night Lights |
| 6 | LANDEIRO, Pedro | Windermere Prep |
NT | 1:02.23 |
| 7 | WILDENBURG, Sean | First Academy (Orlando) |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GIUZIO, Antonio | Windermere Prep |
1:00.73 | Bishop Moore - Carl Friedland Invite 2025 |
| 2 | IVSIC, Miles | First Academy (Orlando) |
56.31 | Lake Buena Vista Home Meet |
| 3 | SAGOTSKY, Alek | Trinity Prep |
54.88 | Winter Park - Brian Jaeger Elite Classic 2025 |
| 4 | HAWLEY, James | Trinity Prep |
54.59 | Bishop Moore - Larry Kelly Invitational 2025 |
| 5 | OKATY, Evan | Lake Highland Prep |
54.70 | Sanford-Seminole Invitational 2025 |
| 6 | WOODSIDE, Jonah | Bishop Moore |
55.32 | Bishop Moore - Larry Kelly Invitational 2025 |
| 7 | SNYDER, Cooper | Bishop Moore |
1:00.18 | ROYAL INVITATIONAL @ The First Academy 2025 |
| 8 | NAVE, Jack | Trinity Prep |
1:03.11 | Bishop Moore - Larry Kelly Invitational 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ROWAN, Dougan | Bishop Moore |
53.37 | Bishop Moore - Larry Kelly Invitational 2025 |
| 2 | DISHMAN, Sean | Lake Highland Prep |
52.83 | Bishop Moore - Larry Kelly Invitational 2025 |
| 3 | ALLEN, Connor | Lake Highland Prep |
51.06 | Lake Highland - Highlander Classic 2025 |
| 4 | COAN, Lucas | Montverde Academy |
49.19 | Mount Dora Christian Invitational #1 |
| 5 | HENLEY, Ayden | Montverde Academy |
49.94 | ROYAL INVITATIONAL @ The First Academy 2025 |
| 6 | CANTERO, Ellijah | Bishop Moore |
51.18 | Bishop Moore - Larry Kelly Invitational 2025 |
| 7 | BROWN, Kamron | Montverde Academy |
52.93 | Mount Dora Christian Invitational #1 |
| 8 | GARDNER, Sawyer | First Academy (Orlando) |
54.48 | Mount Dora Christian Invitational #1 |