| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | SARANITA, Chloe | Windermere Prep |
NT | 28.00 |
| 3 | BASINGER, Lilyana | Windermere Prep |
NT | NT |
| 4 | SULLIVAN, Carrie | Lake Highland Prep |
34.39 | Lake Highland - Highlander Classic 2025 |
| 5 | ZHOU, Lily | Lake Highland Prep |
36.02 | Lake Highland - Highlander Classic 2025 |
| 6 | FERNANDEZ, Natalia | Windermere Prep |
NT | NT |
| 7 | REGAZZI, Sofia | Montverde Academy |
NT | 25.0 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | OSWALD, Mary | Lake Highland Prep |
34.17 | Geneva Classic 2025 |
| 2 | BENNETT, Ailey | Trinity Prep |
33.36 | Bishop Moore - Larry Kelly Invitational 2025 |
| 3 | MOORE, Isabella | Trinity Prep |
31.07 | Mount Dora Christian Invitational #1 |
| 4 | STONE, Abby | Windermere Prep |
30.21 | Windermere Friday Night Lights |
| 5 | HERRINGTON, Blakely | First Academy (Orlando) |
30.86 | ROYAL INVITATIONAL @ The First Academy 2025 |
| 6 | WRIGHT, Stephanie | Lake Highland Prep |
32.46 | Bishop Moore - Larry Kelly Invitational 2025 |
| 7 | ADAMS, Emma | First Academy (Orlando) |
33.80 | Windermere Friday Night Lights |
| 8 | DEBAISE, Anna | Trinity Prep |
34.23 | Hagerty Track and Field Invitational 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MADDALENA, Gabriella | Bishop Moore |
28.33 | Bishop Moore - Larry Kelly Invitational 2025 |
| 2 | SUFFRAN, Engreed | Bishop Moore |
27.69 | Bishop Moore - Larry Kelly Invitational 2025 |
| 3 | LANDRI, Gianna | First Academy (Orlando) |
25.71 | ROYAL INVITATIONAL @ The First Academy 2025 |
| 4 | HUDSON, Nakira | Montverde Academy |
24.40 | ROYAL INVITATIONAL @ The First Academy 2025 |
| 5 | TURNER, Arima | Montverde Academy |
25.29 | ROYAL INVITATIONAL @ The First Academy 2025 |
| 6 | SWEENEY, Londyn | Bishop Moore |
27.61 | Oak Ridge - Pioneer Invitational 2025 |
| 7 | SCHMID, Pearl | First Academy (Orlando) |
27.97 | Ocoee Invitational (Meet is Full) |
| 8 | CANTERO, Elena | Bishop Moore |
28.98 | Lake Highland - Highlander Classic 2025 |