| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | WILLIAMS, Dhamariah | Montverde Academy |
NT | NT 1:03.37 FHSAA 2A Region 2 2023 |
| 2 | REYES, Amara | Bishop Moore |
1:23.46 | Bishop Moore - Larry Kelly Invite 2024 (Regional Qualifier) |
| 3 | MASON, Calista | First Academy (Orlando) |
1:13.29 | Dr. Phillips Panther Invitational |
| 4 | HOLLAND, Jizelle | Montverde Academy |
1:05.28 | Louie Bing Invitational |
| 5 | BROWN, Zahmya | First Academy (Orlando) |
1:06.86 | Winter Park - Wildcat Open 2024 |
| 6 | BONNEWITZ, Paloma | Bishop Moore |
1:15.50 | Bishop Moore - Larry Kelly Invite 2024 (Regional Qualifier) |
| 7 | POPAROD, Kennedy | Bishop Moore |
1:27.68 | Bishop Moore - Larry Kelly Invite 2024 (Regional Qualifier) |
| 8 | BURGOS, Addison | Trinity Prep |
NT | NT |