| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 3 | 286 | FAUSTIN, Christian | JR | Winter Park |
NT | |
| 4 | 837 | WETTELAND, Riley | FR | Windermere Prep |
NT | |
| 5 | 198 | CLARK, Xavier | SO | Winter Park |
NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 833 | SPARTZ, Sean | SO | Windermere Prep |
NT | |
| 2 | 134 | CLARKE, Naeem | SO | University (Orlando) |
NT | |
| 3 | 297 | PONCE-ARCE, Adrian | JR | Trinity Christian (Deltona) |
NT | |
| 4 | 292 | STITES, Nathaniel | SO | Trinity Christian (Deltona) |
1:21.87 | FHSAA 1A Region 2 |
| 5 | 317 | THOMPSON, Ryan | JR | Trinity Christian (Deltona) |
NT | |
| 6 | 748 | HENRY, Kenyon | JR | Jones |
NT | |
| 7 | 287 | ALI, Shariz | SR | Winter Park |
NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 147 | CURRY, Jason | SR | University (Orlando) |
1:06.63 | Dr. Phillips Panther Invitational |
| 2 | 625 | LEWIS, Octavius | FR | Edgewater |
1:04.44 | St. Cloud Invitational |
| 3 | 598 | VECCHIO, Giancarlo | SR | Edgewater |
59.99 | FHSAA 3A District 6 |
| 4 | 148 | MAYERS, Isaiah | JR | University (Orlando) |
57.78 | FHSAA 4A Region 1 |
| 5 | 862 | BOUBE, Mamadou | JR | McIntosh | 59.06 | Satellite High Reggie Johnson Memorial |
| 6 | 428 | MATAMOROS, Elias | JR | Tavares |
1:03.46 | Ocoee High School Preseason Invitational |
| 7 | 298 | PONCE-ARCE, Gabriel | SR | Trinity Christian (Deltona) |
1:05.87 | Holy Trinity Invitational powered by Track Legend |
| 8 | 896 | WILLINGHAM, JJ | SR | McIntosh | 1:07.00 |