| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | McIntosh | B | DNS | |||||
| 3 | Trinity Christian (Deltona) |
A | Thompson | Brittain | Morillo | Cruz | DNS | |
| 4 | Windermere Prep |
A | Delgado | Castanha | Giuzio | Russo | DNS | |
| 5 | Orangewood Christian |
A | Bhullar | Ryckman | Barsoum | Gonzalez | 4:21.66 | FHSAA 1A District 7 |
| 6 | Jones |
A | Hall | Jacobs | Smith | Santlo | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Orlando Christian Prep |
A | rodriguez | angeles | Bolton | Flores | 3:36.96 | 3:36.96 Boone Invitational 2024 |
| 2 | Winter Springs |
A | Johnson | Lowman | Walkin | Williams | 3:35.58 | FHSAA 3A District 6 |
| 3 | University (Orlando) |
A | Mayers | McGinnis | Hafid | Moyer | 3:34.62 | 3:34.62 Metro East Championship |
| 4 | Winter Park |
A | Morrison | Lee | Gamble | Cook | 3:21.13 | FHSAA State Track & Field Championships (4A / Para-Athlete) |
| 5 | Edgewater |
A | Anderson | Collin | Marzolf | Johnson | 3:28.10 | FHSAA 3A District 6 |
| 6 | McIntosh | A | Nowell | Boube | Cromartie | Whitaker | 3:35.00 | |
| 7 | Tavares |
A | Ludwig | Neal | Hartley | Mars | 3:36.71 | 3:36.71 Bears Invitational |
| 8 | Bishop Moore |
A | Talma | Rowan | Woodside | St. Clair | DNF | FHSAA 2A Region 2 |