| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Jones |
A | Corvell | Edouard | Hall | Yarn | 10:37.82 | Sam Burley Hall of Fame Invitational |
| 2 | Bishop Moore |
A | Radford | Yoerger | Delatorore | Lozano | 10:48.00 | |
| 3 | Mount Dora |
A | Vu | Orser | Croeze | Zagorini | 11:16.66 | 2024 Be Like Ike Invitational (meet is full) |
| 4 | Tavares |
A | Lewis | Noll | Noll | Thomas | 11:30.00 | |
| 5 | Trinity Prep |
A | Hennebery | Carlin | Mortimer | Jessen | NT | |
| 6 | Lake Highland Prep |
A | Tomasic | Johnson | Stanton | Shah | NT |