| Lane | # | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|---|
| 3 | 4 | LUNA, Allison | SO | Lake Region | 1:26.89 | Lake Region Distance Carnival 2022 | 
| 4 | 73 | BROWN, Laila | JR | Frostproof | 1:06.75 | Polk County Championship 2019 | 
| 5 | 39 | STUBBS, Rihanna | SO | Lake Region | 1:08.96 | Lake Region Distance Carnival 2022 |