| Lane | # | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|---|
| 1 | 136 | JACKSON, Jaquan | 8 | Frostproof | NT | |
| 2 | 132 | PITTMAN, Daiveon | SO | Frostproof | 25.49 | Lake Region Sprint/Hurdle Meet 2022 | 
| 3 | 87 | WILLIAMS, Davieon | SR | Frostproof | 24.54 | Lake Region Sprint/Hurdle Meet 2022 | 
| 4 | 61 | SMITH, Jordan | SO | Davenport | 24.00 | |
| 5 | 60 | PIERRE, Isaiah | SO | Davenport | 24.00 | |
| 6 | 68 | AGYEDU, Samuel | SO | Davenport | 25.00 | |
| 7 | 95 | COBB, Antwan | 7 | Frostproof | 33.24 | Lake Region Sprint/Hurdle Meet 2022 |