| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Kathleen |
A | Hillary | Philogene | Sherwood | Tunis | NT | |
| 4 | Ridge Community |
A | Evans | Williams | Patrick | Allen | 54.92 | Poinciana Relay Meet |
| 5 | Discovery |
A | Brown-Newman | Alcius | Green | Newton | 58.45 | Poinciana Relays |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Davenport |
A | Clemente | Clemente | Monestine | Page | 51.26 | 51.26 Polk County Championship 2023 |
| 2 | Auburndale |
A | Parrish | Daughtry | Forsett | Rogers | 50.10 | |
| 3 | Cocoa |
A | Williams | Parham | Edgecombe | Henderson | 50.00 | |
| 4 | Lake Wales |
A | St. Fleur | Bonds | Leverston | Walker | 49.00 | |
| 5 | Dr. Phillips |
A | Castell | Martin | Rene | Smith | 49.61 | St. Cloud Invitational |
| 6 | Bartow |
A | Hendrix | Curry | Brisbane | Denson | 50.06 | |
| 7 | Frostproof |
A | Vasquez | Ingraham | McNeil | Smith | 51.25 | 51.25 Polk County Championship 2023 |