| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Davenport |
A | Clemente | Neal | Clemente | Page | NT | |
| 4 | Discovery |
A | Buckle | Buckle | Leavengood | Buckle | 6:07.53 | Poinciana Relays |
| 5 | Tenoroc |
A | Wilson | Jean | Fernandez | Smith | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ridge Community |
A | Weekes | Patrick | Head | Daltirus | DNS | |
| 2 | Bartow |
A | Veney | Tucker | Johnson | McCants | 4:26.97 | |
| 3 | Cocoa |
A | Barnes | Jacavage | Harp | Gordon | 4:10.00 | |
| 4 | Lake Wales |
A | Bonds | Walker | Henry | St. Fleur | 4:05.00 | |
| 5 | Dr. Phillips |
A | Kirkland | Smith | Caceres | Heredia | 4:05.99 | Sam Burley Hall of Fame Invitational |
| 6 | East Ridge |
A | Terrio | Benson | Terrio | Mustin | 4:18.00 | |
| 7 | Auburndale |
A | Parrish | Daughtry | Boone | Rogers | 4:27.01 | Winter Haven Classic |