| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Orangewood Christian |
A | Aponte | Baylon | Evers | Classon | DNS | |
| 5 | Lake Brantley |
A | Atkinson | Boutwell | Joseph | Maximin | 49.95 | 49.95 Sanford - Seminole COACH ERNEST MCPHERSON INVITATIONAL 2024 |
| 6 | Winter Park |
A | Eady | Hamilton | Leon | Phillips | DQ | 57.03 East River Developmental Meet |