| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Boone |
A | McNulty | Cannella | Gill | Williams | DNS | FHSAA Class 4A Track & Field State Championships |
| 2 | Winter Park |
A | Washington | Escandon | Rodriguez | Osburn | 8:25.80 | Metro East Championship |
| 3 | Lake Highland Prep |
A | Allen | Choung | Acomb | Fineman | 8:34.16 | 2024 Spring Break Bash |
| 4 | Tohopekaliga |
A | Francis | Lopez | Boone | Allen | 9:01.72 | Dr. Phillips Panther Invitational |
| 5 | Jones |
A | Hall | Collins | Horne | Hall | 9:22.07 | Metro East Championship 2024 |
| 6 | Orangewood Christian |
A | Hanna | Erwin | Platts | Ryckman | NT |