| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | 
|---|---|---|---|---|---|---|---|---|
| 1 |  Boone | 
		A | McNulty | Cannella | Gill | Williams | DNS | FHSAA Class 4A Track & Field State Championships | 
| 2 |  Winter Park | 
		A | Washington | Escandon | Rodriguez | Osburn | 8:25.80 | Metro East Championship | 
| 3 |  Lake Highland Prep | 
		A | Allen | Choung | Acomb | Fineman | 8:34.16 | 2024 Spring Break Bash | 
| 4 |  Tohopekaliga | 
		A | Francis | Lopez | Boone | Allen | 9:01.72 | Dr. Phillips Panther Invitational | 
| 5 |  Jones | 
		A | Hall | Collins | Horne | Hall | 9:22.07 | Metro East Championship 2024 | 
| 6 |  Orangewood Christian | 
		A | Hanna | Erwin | Platts | Ryckman | NT |