| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 647 | MANTLOW, Alicia | SO | Lake Brantley |
NT | |
| 2 | 840 | MOMPREMIER, Lea | JR | Tohopekaliga |
1:19.67 | Orange Belt Conference Championships |
| 3 | 1 | HINSON, Saraya | JR | Mainland |
1:18.13 | 5-Star Conference Championship (FHSAA Sanctioned Meet) |
| 4 | 795 | DENNARD, Makayla | JR | Jones |
1:17.51 | Metro East Championship 2024 |
| 5 | 501 | WAHL, Alyssa | SR | Oviedo |
1:10.00 | |
| 6 | 767 | BURGOS, Addison | JR | Trinity Prep |
1:16.75 | |
| 7 | 799 | HUNTER, Kimora | SR | Jones |
1:17.96 | Metro East Championship 2024 |
| 8 | 808 | BRYANT, Brooklyn | JR | Jones |
1:18.34 | Metro East Championship 2024 |
| 9 | 166 | EVERS, Mercedes | SO | Orangewood Christian |
NT |