| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | LANE, Claudia | Winter Park | NT | |
| 4 | LLANOS, Darieliz | Boone | NT | |
| 5 | BARTIMUS, Emily | Winter Park | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MANNING, Lakisha | Boone | NT | |
| 2 | HARDEN, Trinity | Orlando Christian Prep | NT | |
| 3 | DENNEY, Olivia | Winter Park | 1:03.16 | St. Cloud Track Invitational |
| 4 | UGWUOKE, Hope | Timber Creek | NT | |
| 5 | BURGESS, Liana | Boone | NT | |
| 6 | CLEMONS, Caitlin | Winter Park | NT | |
| 7 | BARRERO, Annie | Boone | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | OVERBY, Nia | Boone | NT | |
| 2 | QUASHIE, Makalya | Orlando Christian Prep | 1:19.71 | Mount Dora Christian Invitational Meet #1 |
| 3 | CHAMBERS, Jayla | Boone | 1:16.72 | Metro East Conference Championships |
| 4 | WILLIAMS, Mykel | Boone | 1:09.65 | Dr. Phillips Panther Invite |
| 5 | MAPILI, Talia | Timber Creek | 1:10.62 | Metro East Conference Championships |
| 6 | LEWIS, Aaliyah | Orlando Christian Prep | 1:19.02 | Mount Dora Developmental Meet #2 |
| 7 | COOPER, Brianna | Orlando Christian Prep | 1:32.19 | Mount Dora Christian Developmental Meet #3 |
| 8 | LOPEZ, Stephanie | Timber Creek | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | RODDA, Brooke | Timber Creek | 1:07.63 | Metro East Conference Championships |
| 2 | DAY, Jani | Winter Park | 1:04.97 | Dr. Phillips Panther Invite |
| 3 | OQUENDO, Eliana | Winter Park | 1:03.22 | Metro East Conference Championships |
| 4 | KELLY, Jade | Boone | NT | |
| 5 | BROWN, Siarah | Orlando Christian Prep | 1:03.22 | Dr. Phillips Panther Invite |
| 6 | BERNARD, Patley | Winter Park | 1:04.89 | Metro East Conference Championships |
| 7 | GUNN, Aalyssa | Orlando Christian Prep | 1:06.37 | Highlander Classic 2022 |
| 8 | ZELLA, Alexandria | Boone | 1:08.42 | Metro East Conference Championships |